Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry

William Bauta; Marcia Grayson; Rossella Titone; Jennifer Rebeles; Vivienne I. Rebel

doi:10.3791/65294

Journal

化学

Porphyrin-Modified Beads in Flow Cytometry

March 24, 2023

Author Spotlight: Porphyrin-Modified Beads for Use as Compensation Controls in Flow Cytometry

01:03 min

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1,282 Views

Preparation of Meso-Tetra(4-Carboxyphenyl) Porphine (TCPP) Stock Solution

01:33 min

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112 Views

Combining Amine-Functionalized Polystyrene Beads with TCPP Solution

01:00 min

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121 Views

Labeling Amine-Functionalized Polystyrene Beads with TCPP

02:13 min

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97 Views

Quality Check (QC) of the TCPP Beads by Flow Cytometry

01:58 min

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87 Views

TCPP Bead Filtration

02:56 min

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124 Views

William Bauta¹, Marcia Grayson¹, Rossella Titone¹, Jennifer Rebeles¹, Vivienne I. Rebel¹

¹bioAffinity Technologies

Summary

The protocol describes how porphyrin-based compensation beads for flow cytometry are prepared by the reaction of amine-functionalized polystyrene beads with the porphyrin TCPP and the amide coupling reagent EDC. A filtration procedure is used to reduce the particulate byproducts.