Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

January 12, 2024

Author Spotlight: Advancing the Use of Tissue Chip Technology for Studying Human Tissues

µSiM Device Assembly for Blood Brain Barrier Monoculture

Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis

Molly C. McCloskey¹, Pelin Kasap², Michelle Trempel¹, Louis P. Widom³, Julia Kuebel¹, Kaihua Chen¹, Thomas R. Gaborski³, Britta Engelhardt², James L. McGrath¹

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

Summary

This report provides protocols for assembly, cell culture, and assays on the µSiM platform for the construction of blood-brain barrier models.

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