Quantification of Adeno-Associated Viral Genomes in Purified Vector Samples by Digital Droplet Polymerase Chain Reaction

Nathalie Van den Berghe; Elien Costermans; Samir Nuseibeh; Tine Brouns; Inge Van Hove; Benjamien Moeyaert; Els Henckaerts

doi:10.3791/67252

Journal

Bioengineering

Standardized Protocol for Viral Genome Quantification Using Digital Droplet PCR

October 11, 2024

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

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Nathalie Van den Berghe¹, Elien Costermans¹, Samir Nuseibeh¹, Tine Brouns¹, Inge Van Hove¹, Benjamien Moeyaert¹, Els Henckaerts¹

¹Trellis Research Group, Department of Cellular and Molecular Medicine, Department of Microbiology, Immunology and Transplantation,KU Leuven

Summary

Precise quantification of adeno-associated virus (AAV) vector genome copies is critical, but a standardized protocol has yet to be established. This protocol describes a validated method for preparing purified AAV samples and conducting digital droplet polymerase chain reaction (dd_PCR) to reliably quantify the viral genome titer.

Standardized Protocol for Viral Genome Quantification Using Digital Droplet PCR

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

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