Journal
Medicine
SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing

SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing

May 10, 2024

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

01:35 min
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193 Views

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

01:37 min
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54 Views

Purification of Ligation Product, Amplification and Library Purification for Multi-Gene SNP Detection in Gastric Cancer

03:16 min
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56 Views
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1Nanfang Hospital, Southern Medical University, 2Research and Development Department,Guangzhou Darui Biotechnology Co., Ltd, 3Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology,Southern Medical University, 4Provincial Key Laboratory of Immune Regulation and Immunotherapy,Southern Medical University

Summary

English

Automatically Generated

This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.

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