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Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining
December 22, 2023
細胞周期のG1期における一本鎖DNA病巣の可視化
01:21 min
•
遺伝毒性ストレスによるssDNA病巣形成を測定するためのG1同期細胞の調製
07:11 min
•
Qingyue Zhang
2
,
Marc A. Kerzhnerman
2
,
Nelson García-Vázquez
2,3
,
Gergely Rona
2,4,5
1
Department of Biochemistry and Molecular Pharmacology
,
NYU Grossman School of Medicine
,
2
The Laura and Isaac Perlmutter Cancer Center
,
NYU Langone Health
,
3
Department of Cell Biology
,
NYU Grossman School of Medicine
,
4
Howard Hughes Medical Institute
,
NYU Grossman School of Medicine
,
5
Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences
,
HUN-REN Research Centre for Natural Sciences
Summary
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次のプロトコルはRPA2 immunofluorescent stainingに先行している細胞周期の同期を利用して細胞周期のG1段階のsingle-stranded DNAの焦点の検出を示す。
Article