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Enhancing EV Isolation for Precise Biofluid Analysis
October 27, 2023
基于化学亲和力的生物流体细胞外囊泡分离用于蛋白质组学和磷酸化蛋白质组学分析
01:49 min
•
使用 EVtrap 方法从人尿液样本中分离细胞外囊泡 (EV)
01:54 min
•
用于蛋白质组学和磷酸化蛋白质组学分析的干燥细胞外囊泡 (EV) 样品的制备
02:56 min
•
使用LC-MS/MS对细胞外囊泡(EV)样品进行蛋白质组/磷酸化蛋白质组分析
02:58 min
•
Yi-Kai Liu
,
Zhuojun Luo
,
Anton Iliuk
2
,
Weiguo Andy Tao
2,3,4
1
Department of Biochemistry
,
Purdue University
,
2
Tymora Analytical Operations
,
3
Department of Chemistry
,
Purdue University
,
4
Purdue Institute for Cancer Research
,
Purdue University
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本方案为利用功能化磁珠有效分离尿细胞外囊泡提供了详细的描述。此外,它还包括后续分析,包括蛋白质印迹、蛋白质组学和磷酸化蛋白质组学。
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