Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Haixia Xu; Junyan Lu; Zhou Li; Ran Chen

doi:10.3791/65647

Optimizing Low-Frequency Mutation Detection with Wild-Type Blocking PCR

August 23, 2024

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

00:00 min

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Haixia Xu*¹, Junyan Lu*², Zhou Li, Ran Chen

¹Kindstar Global Technology Inc., ²Yongzhou Central Hospital

Summary

This article introduces the application of a low-frequency detection method based on Sanger sequencing in angioimmunoblastic lymphoma. Provide a basis for applying this method to other diseases.

Optimizing Low-Frequency Mutation Detection with Wild-Type Blocking PCR

Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation

Summary

Article