<em>In Vitro</em> Model of Fetal Human Vessel On-chip to Study Developmental Mechanobiology

Telma Ventura; Ewan Joshua Egan; Nicola Roman&#242;; Antonella Fidanza

doi:10.3791/65492

Journal

Developmental Biology

Differentiation and Mechanical Stimulation of hiPSC-Derived Endothelial Cells

July 28, 2023

Author Spotlight: Studying hiPSC-Derived Endothelial Cells Cultured Under Fluidic-Mediated Mechanical Stimulation

01:16 min

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573 Views

Differentiation of hiPSCs into Endothelial Cells

04:54 min

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126 Views

Seeding CD34+ Cells into Fluidic Chips

01:16 min

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94 Views

Application of Continuous Flow to Endothelial Cells: Aorta-on-a-Chip

02:12 min

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104 Views

Telma Ventura¹, Ewan Joshua Egan¹, Nicola Romanò², Antonella Fidanza¹

¹Centre for Regenerative Medicine, Institute for Regeneration and Repair,University of Edinburgh, ²Centre for Discovery Brain Sciences,University of Edinburgh

Summary

Described here is a simple workflow to differentiate endothelial cells from human pluripotent stem cells followed by a detailed protocol for their mechanical stimulation. This allows for the study of the developmental mechanobiology of endothelial cells. This approach is compatible with downstream assays of live cells collected from the culture chip after mechanical stimulation.