Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy

Perla P&#233;rez-Trevi&#241;o; Bianca Nieblas; Selma Romina L&#243;pez-Vaquera; Noem&#237; Garc&#237;a

doi:10.3791/65306

Journal

Biology

Confocal Microscopic Analysis of Rat Muscle Mitochondria

December 01, 2023

Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

01:43 min

•

1,220 Views

Rat Gastrocnemius Muscle Fiber Bundle Dissection

01:06 min

•

345 Views

Confocal Live Cell Imaging of Rat Skeletal Muscle Mitochondria

03:04 min

•

256 Views

Mitochondrial Density Analysis

02:28 min

•

213 Views

Mitochondrial Distribution Analysis Using Fast Fourier Transform

02:47 min

•

190 Views

Perla Pérez-Treviño¹, Bianca Nieblas^1,2, Selma Romina López-Vaquera¹, Noemí García^1,2,3

¹Experimental Medicine and Advanced Therapies, The Institute for Obesity Research,Tecnologico de Monterrey, ²Escuela de Medicina y Ciencias de la Salud,Tecnologico de Monterrey, ³Preclinical Research Unit,Tecnologico de Monterrey

Summary

Here, we present a protocol to analyze changes in mitochondrial density and longitudinal distribution by live-skeletal muscle imaging using confocal microscopy for mitochondrial network scanning.