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High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
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March 12, 2012
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Two distinct methods to screen plants with root-knot nematodes are described. The described approaches include high-throughput screens with nematodes in a nondestructive manner facilitating the use of these plants in breeding programs.
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Root-knot NematodesMeloidogyne Spp.High-throughput ScreensPlant ParasitesObligate ParasitesEconomically Important NematodesSecond-stage Juvenile (J2)Infective StageSoil MovementRoot PenetrationFeeding Site EstablishmentGiant CellsGall FormationRoot Knot SymptomMoltsAdult StageEgg LayingLife Cycle CompletionTraditional ProtocolPlant InfectionHigh-throughput Assays
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Atamian, H. S., Roberts, P. A., Kaloshian, I. High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.. J. Vis. Exp. (61), e3629, doi:10.3791/3629 (2012).
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