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Biochemistry
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Optimizing FPLC for Efficient Purification of His-Tagged FEN1 Protein
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Hızlı Protein Sıvı Kromatografi Sistemi Kullanılarak 6X His Etiketli Bir Proteinin Afinite Saflaştırılması
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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Hızlı Protein Sıvı Kromatografi Sistemi Kullanılarak 6X His Etiketli Bir Proteinin Afinite Saflaştırılması

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01:19 min

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April 26, 2024

DOI:

10.3791/66529-v

DOI:
10.3791/66529-v

•

01:19 min
April 26, 2024

•

14 Views
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1Department of Biology,Indiana University Indianapolis

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6X-His-tagged ProteinAffinity PurificationFast Protein Liquid Chromatography (FPLC)Flap Endonuclease 1 (FEN1)Protein ExpressionProtein PurificationProtein StabilityProtein FunctionalitySample PreparationColumn PackingFlow RateElution ParametersChromatogram AnalysisRecombinant Protein Storage

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Sridharan, M., Battapadi, T., Balakrishnan, L. Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System . J. Vis. Exp. (206), e66529, doi:10.3791/66529 (2024).

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