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Developmental Biology
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Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
/
生きたマウス精子における先体カルシウム動態とエキソサイトーシスのリアルタイムイメージング
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JoVE Journal
Developmental Biology
Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm
Please note that all translations are automatically generated.
Click here for the English version.
生きたマウス精子における先体カルシウム動態とエキソサイトーシスのリアルタイムイメージング
Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
DOI:
10.3791/65962-v
•
00:46 min
•
October 13, 2023
•
Roy Cohen
2
,
Danielle M. Sosnicki
,
Melissa A. White
,
Jacquelyn L. Nelson
,
Chinatsu Mukai
,
Alexander J. Travis
2
1
Baker Institute for Animal Health, College of Veterinary Medicine
,
Cornell University
,
2
Department of Public and Ecosystem Health, College of Veterinary Medicine
,
Cornell University
Tags
Acrosome Exocytosis
Sperm
Calcium Signaling
Capacitation
Fertilization
AcroSensE
Genetically Encoded Calcium Indicator
GCaMP
MCherry
High Frame-rate Imaging
Stimulant Delivery System
Genetic Tools
CRISPR
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生きたマウス精子における先体カルシウム動態とエキソサイトーシスのリアルタイムイメージング
リアルタイムイメージングのためのAcroSensEマウスモデルからの精子の採取と洗浄
マウス精子の生細胞モニタリングのための単一細胞刺激剤送達システムのセットアップ(英語)
顕微鏡と覚醒剤送達システムを組み合わせて、AcroSensEモデルマウスの生精子をモニタリング
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