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A Preparação Ex Vivo do Corte da Medula Espinhal para o Registro de Patch-Clamp de Células Inteiras em Neurônios Motores Durante a Estimulação da Medula Espinhal
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Neuroscience
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JoVE Journal Neuroscience
The Ex vivo Preparation of Spinal Cord Slice for the Whole-Cell Patch-Clamp Recording in Motor Neurons During Spinal Cord Stimulation

A Preparação Ex Vivo do Corte da Medula Espinhal para o Registro de Patch-Clamp de Células Inteiras em Neurônios Motores Durante a Estimulação da Medula Espinhal

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02:04 min

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September 08, 2023

DOI:

10.3791/65385-v

DOI:
10.3791/65385-v

•

02:04 min
September 08, 2023

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7 Views
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1National Engineering Research Center of Neuromodulation, School of Aerospace Engineering,Tsinghua University, 2IDG/McGovern Institute for Brain Research,Tsinghua University, 3School of Life Sciences,Tsinghua University

Tags

Spinal Cord StimulationMotor NeuronsPatch-clamp RecordingEx Vivo Spinal Cord SliceCell ViabilityElectrophysiologyAction PotentialsSpinal Cord InjurySensorimotor BehaviorsSingle-cell Recording

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Yao, Q., Luo, X., Liu, J., Li, L. The Ex vivo Preparation of Spinal Cord Slice for the Whole-Cell Patch-Clamp Recording in Motor Neurons During Spinal Cord Stimulation. J. Vis. Exp. (199), e65385, doi:10.3791/65385 (2023).

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