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Påvisning og kvantifisering av kalsitoningenrelatert peptid (CGRP) i humant plasma ved bruk av en modifisert enzymbundet immunosorbentanalyse
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1Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine
Chapters
- 00:04Introduction
- 01:03Collection, Preparation, and Extraction of Plasma Sample
- 03:28ELISA Plate Preparation (Blocking)
- 04:16Enzyme-Linked Immunosorbent Assay (ELISA)
- 06:06Results: Effect of Blocking on CGRP Quantification
- 06:29Conclusion
Publiserte data vedrørende kalsitoningenrelaterte peptidkonsentrasjoner (CGRP) i humant plasma er inkonsistente. Disse inkonsekvensene kan skyldes mangelen på en standardisert, validert metodikk for å kvantifisere dette nevropeptidet. Her beskriver vi en validert ELISA-protokoll (enzyme-linked immunosorbent assay) for å rense og kvantifisere CGRP i humant plasma.
