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Engineering onkogene heterozygote gain-of-function mutasjoner i humane hematopoietiske stamceller og stamceller
This content is Free Access.
1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz
Chapters
- 00:05Introduction
- 00:54sgRNA Design
- 01:57Homology-Directed Repair Vector Construction
- 05:42Editing of Hematopoietic Stem and Progenitor Cells
- 09:48Results: Heterozygous Gain-of-Function Mutations in Hematopoietic Stem and Progenitor Cells by Combining the Use of CRISPR/Cas9 and Dual rAAV Donor Transduction
- 11:19Conclusion
Nye strategier for å trofast modellere somatiske mutasjoner i hematopoietiske stam- og stamceller (HSPCs) er nødvendige for å bedre studere hematopoietisk stamcellebiologi og hematologiske maligniteter. Her beskrives en protokoll for å modellere heterozygote gain-of-function-mutasjoner i HSPC-er ved å kombinere bruken av CRISPR/Cas9 og dobbel rAAV-donortransduksjon.
