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Bestemmelse af in vitro - og cellulær tændingskinetik til fluorogene RNA-aptamerer

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Bestemmelse af in vitro - og cellulær tændingskinetik til fluorogene RNA-aptamerer

1,584 Views

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08:11 min

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August 09, 2022

DOI:

10.3791/64367-v

DOI:

10.3791/64367-v

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08:11 min

August 09, 2022

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4 Views

Madeline M. Mumbleau*¹, Madeline R. Meyer*¹, Ming C. Hammond

¹Department of Chemistry and Henry Eyring Center for Cell & Genome Science,University of Utah

Tags

Fluorogenic RNA Aptamers In Vitro Kinetics Cellular Kinetics Real-time Studies RNA Renaturation Thermocycler Plate Reader Injector Fluorescence Measurements Binding Buffer D-FHBI

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