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Geração de Microambientes Celulares Multicue por UV-Fotopatterning de Substratos de Cultura Celular Tridimensional
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Geração de Microambientes Celulares Multicue por UV-Fotopatterning de Substratos de Cultura Celular Tridimensional

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2,162 Views

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09:30 min

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June 02, 2022

DOI:

10.3791/63988-v

DOI:
10.3791/63988-v

•

09:30 min
June 02, 2022

•

1 Views
, , , , ,
1Department of Biomedical Engineering,Eindhoven University of Technology, 2Institute for Complex Molecular Systems,Eindhoven University of Technology

Summary

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Tradicionalmente, a cultura celular é realizada em substratos planares que imitam mal o ambiente natural das células in vivo. Aqui descrevemos um método para produzir substratos de cultura celular com geometrias curvas fisiologicamente relevantes e proteínas extracelulares micropatteradas, permitindo investigações sistemáticas sobre o sensoriamento celular dessas pistas extracelulares.

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Cite this Article

van der Putten, C., D’Urso, M., Bril, M., Woud, T. E., Bouten, C. V. C., Kurniawan, N. A. Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates. J. Vis. Exp. (184), e63988, doi:10.3791/63988 (2022).

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