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原位 冷冻切片斑马鱼胚胎中的杂交结合免疫组织化学
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Neuroscience
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JoVE Journal Neuroscience
In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos
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原位 冷冻切片斑马鱼胚胎中的杂交结合免疫组织化学

English

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2,327 Views

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07:36 min

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March 03, 2022

DOI:

10.3791/63715-v

DOI:
10.3791/63715-v

•

07:36 min
March 03, 2022

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2 Views
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1School of Life Sciences,Nantong University, 2Medical School,Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration,Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center,Nantong University

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In Situ HybridizationImmunohistochemistryCryosectionedZebrafish EmbryosMRNAProtein AnalysisFixationDehydrationRehydrationProteinase K DigestionPre-hybridizationHybridizationProbeWashingSSCTSSC

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Wang, J., Chai, R., Fang, X., Gu, J., Xu, W., Chen, Q., Chen, G., Zhu, S., Jin, Y. In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos. J. Vis. Exp. (181), e63715, doi:10.3791/63715 (2022).

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