Using transgenic fluorescent mice, detailed protocols are described to assess in vivo axonal transport of signaling endosomes and mitochondria within motor and sensory axons of the intact sciatic nerve in live animals.
Tosolini, A. P., Villarroel-Campos, D., Schiavo, G., Sleigh, J. N. Expanding the Toolkit for In Vivo Imaging of Axonal Transport. J. Vis. Exp. (178), e63471, doi:10.3791/63471 (2021).