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Mangesidet massespektrometrisk undersøgelse af neuropeptider i Callinectes sapidus
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Chemistry
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JoVE Journal Chemistry
Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Mangesidet massespektrometrisk undersøgelse af neuropeptider i Callinectes sapidus

English

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2,285 Views

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09:22 min

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May 31, 2022

DOI:

10.3791/63322-v

DOI:
10.3791/63322-v

•

09:22 min
May 31, 2022

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2 Views
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1Department of Chemistry,University of Wisconsin-Madison, 2School of Pharmacy,University of Wisconsin-Madison

Summary

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Massespektrometrisk karakterisering af neuropeptider giver sekvens-, kvantations- og lokaliseringsinformation. Denne optimerede arbejdsgang er ikke kun nyttig til neuropeptidundersøgelser, men også andre endogene peptider. Protokollerne her beskriver prøveforberedelse, MS-erhvervelse, MS-analyse og databasegenerering af neuropeptider ved hjælp af LC-ESI-MS, MALDI-MS-spotting og MALDI-MS-billeddannelse.

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Phetsanthad, A., Vu, N. Q., Li, L. Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus. J. Vis. Exp. (183), e63322, doi:10.3791/63322 (2022).

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