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Residu-specifieke uitwisseling van Proline door Proline-analogen in fluorescerende eiwitten: hoe "moleculaire chirurgie" van de ruggengraat de vouwing en stabiliteit beïnvloedt
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How “Molecular Surgery” of the Backbone Affects Folding and Stability
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Residu-specifieke uitwisseling van Proline door Proline-analogen in fluorescerende eiwitten: hoe "moleculaire chirurgie" van de ruggengraat de vouwing en stabiliteit beïnvloedt

Nederlands

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2,653 Views

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10:31 min

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February 03, 2022

DOI:

10.3791/63320-v

DOI:
10.3791/63320-v

•

10:31 min
February 03, 2022

•

17 Views
, , , ,
1Institute of Chemistry,Technische Universität Berlin, 2Department of Chemistry,University of Manitoba, 3Institute of Physics,Martin-Luther-Universität Halle-Wittenberg

Summary

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Om de beperkingen van klassieke site-directed mutagenese te overwinnen, werden proline analogen met specifieke modificaties opgenomen in verschillende fluorescerende eiwitten. We laten zien hoe de vervanging van waterstof door fluor of van de enkele door dubbele bindingen in prolineresiduen ("moleculaire chirurgie") fundamentele eiwiteigenschappen beïnvloedt, inclusief hun vouwing en interactie met licht.

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Cite this Article

Thi To, T. M., Kubyshkin, V., Schmitt, F., Budisa, N., Friedrich, T. Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability. J. Vis. Exp. (180), e63320, doi:10.3791/63320 (2022).

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