Journal
/
Biochemistry
/
In vitro Karakterisering van Histone Chaperones met behulp van Analytische, Pull-Down en Chaperoning Assays
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Please note that all translations are automatically generated. Click here for the English version.

In vitro Karakterisering van Histone Chaperones met behulp van Analytische, Pull-Down en Chaperoning Assays

Nederlands

Automatically Generated

2,546 Views

•

08:16 min

•

December 29, 2021

DOI:

10.3791/63218-v

DOI:
10.3791/63218-v

•

08:16 min
December 29, 2021

•

8 Views
, , , , , , , , ,
1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology,KIIT University, 4Department of Molecular Biophysics and Biochemistry,Yale University, 5Department of Pharmacology,University of Vermont College of Medicine

Summary

Automatically generated

Dit protocol beschrijft een reeks methoden die analytische grootte-uitsluitingschromatografie omvatten om histon chaperone oligomerisatie en stabiliteit te bestuderen, pull-down assay om histon chaperone-histon interacties te ontrafelen, AUC om de stoichiometrie van de eiwitcomplexen te analyseren, en histon chaperoning assay om functioneel een vermeende histon chaperone in vitro te karakteriseren.

Explore More Videos

Histone ChaperonesPull-down AssaysAnalytical Size Exclusion ChromatographyAnalytical Ultra-centrifugationHistone Chaperoning AssayChromatin ResearchH2A/H2B DimerH3/H4 TetramerSDS-PAGECoomassie Brilliant Blue R250Dialysis BufferAnalytical Ultra Centrifuge

Read Article

Cite this Article

Bobde, R. C., Saharan, K., Baral, S., Gandhi, S., Samal, A., Sundaram, R., Kumar, A., Singh, A. K., Datta, A., Vasudevan, D. In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays. J. Vis. Exp. (178), e63218, doi:10.3791/63218 (2021).

Download .ris file

Copy

Share Video

.

Copy