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Generazione di transgenici e knockout in specie di Strongyloides mediante microiniezione
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
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Generazione di transgenici e knockout in specie di Strongyloides mediante microiniezione

italiano

Automatically Generated

2,396 Views

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09:42 min

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October 07, 2021

DOI:

10.3791/63023-v

DOI:
10.3791/63023-v

•

09:42 min
October 07, 2021

•

5 Views
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1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

Summary

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Il toolkit genomico funzionale per i nematodi parassiti Strongyloides stercoralis e Strongyloides ratti comprende transgenesi, mutagenesi mediata da CRISPR/Cas9 e RNAi. Questo protocollo dimostrerà come utilizzare la microiniezione intragonennale per introdurre transgeni e componenti CRISPR in S. stercoralis e S. ratti.

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Cite this Article

Castelletto, M. L., Hallem, E. A. Generating Transgenics and Knockouts in Strongyloides Species by Microinjection. J. Vis. Exp. (176), e63023, doi:10.3791/63023 (2021).

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