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Het genereren van transgenen en knock-outs in strongyloides soorten door micro-injectie
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
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Het genereren van transgenen en knock-outs in strongyloides soorten door micro-injectie

Nederlands

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2,396 Views

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09:42 min

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October 07, 2021

DOI:

10.3791/63023-v

DOI:
10.3791/63023-v

•

09:42 min
October 07, 2021

•

5 Views
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1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

Summary

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De functionele genomische toolkit voor de parasitaire nematoden Strongyloides stercoralis en Strongyloides ratti omvat transgenese, CRISPR/Cas9-gemedieerde mutagenese en RNAi. Dit protocol zal demonstreren hoe intragonadale micro-injectie kan worden gebruikt om transgenen en CRISPR-componenten in S. stercoralis en S. ratti te introduceren.

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Cite this Article

Castelletto, M. L., Hallem, E. A. Generating Transgenics and Knockouts in Strongyloides Species by Microinjection. J. Vis. Exp. (176), e63023, doi:10.3791/63023 (2021).

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