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Hjerneorganoidgenerering fra inducerede pluripotente stamceller i hjemmelavede minibioreaktorer
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
DOI:

10:16 min

December 11, 2021

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Chapters

  • 00:02Introduction
  • 01:41Transforming Petri Dishes Into Mini Bioreactors
  • 02:51Induction of Neuronal Differentiation of iPSCs
  • 03:51The Formation of Spheroids from Neuroepithelial Precursor Cells
  • 05:38Obtaining and Cultivation of Organoids
  • 07:59Results
  • 09:19Conclusion

Summary

Automatic Translation

Her beskriver vi en protokol til generering af hjerneorganoider fra menneskeskabte pluripotente stamceller (iPSC'er). For at opnå hjerneorganoider i store mængder og af høj kvalitet bruger vi hjemmelavede minibioreaktorer.

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