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使用遗传编码的Ca^{2 +} 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca^{2 +} 摄取进行成像

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

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使用遗传编码的Ca^{2 +} 指示剂（GECI）对星形胶质细胞和神经元中的线粒体Ca^{2 +} 摄取进行成像

3,824 Views

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07:46 min

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January 22, 2022

DOI:

10.3791/62917-v

DOI:

10.3791/62917-v

•

07:46 min

January 22, 2022

•

8 Views

Nannan Zhang¹, Zhe Zhang^1,2, Ilker Ozden², Shinghua Ding^1,2

¹Dalton Cardiovascular Research Center,University of Missouri-Columbia, ²Department of Biomedical, Biological and Chemical Engineering,University of Missouri-Columbia

Summary

该proctocol旨在为星形胶质细胞和神经元 中的体外 和体内线粒体Ca^{2 +} 成像提供一种方法。

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