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September 08, 2021
DOI:
1CIRI, Centre International de Recherche en Infectiologie, GIMAP team,University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, 2Department of Infectious Agents and Hygiene,University Hospital of St-Etienne, St-Etienne, France, 3Department of Infectious Diseases,University Hospital of St-Etienne, St-Etienne, France, 4CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team,University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, 5Department of Bacteriology, Institute for Infectious Agents,Hospices Civils de Lyon, Lyon, France
Summary
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Este protocolo tiene como objetivo describir cómo estudiar el alcance de la internalización de Staphylococcus aureus y su capacidad para sobrevivir dentro de la célula huésped humana, así como la eficacia intracelular de los compuestos antimicrobianos.
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Rigaill, J., Audoux, E., Rodriguez, K., Peyron, A., Berthelot, P., Josse, J., Laurent, F., Caire, R., Verhoeven, P. O. Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds. J. Vis. Exp. (175), e62903, doi:10.3791/62903 (2021).
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