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07:58 min
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October 07, 2021
DOI:
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
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Dieses Protokoll beschreibt einen effizienten und reproduzierbaren Ansatz für histologische Studien des Mausgehirns, einschließlich Perfusion, Hirnschnitt, frei schwebende Immunfärbung, Gewebemontage und Bildgebung.
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Cite this Article
Tu, L., Zhang, N., Conde, K. M., Bean, J. C., Wang, C., Xu, Y. Free-floating Immunostaining of Mouse Brains. J. Vis. Exp. (176), e62876, doi:10.3791/62876 (2021).
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