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07:58 min
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October 07, 2021
DOI:
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
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Ce protocole décrit une approche efficace et reproductible pour les études histologiques du cerveau de souris, y compris la perfusion, la section du cerveau, l’immunocoloration flottante, le montage tissulaire et l’imagerie.
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Tu, L., Zhang, N., Conde, K. M., Bean, J. C., Wang, C., Xu, Y. Free-floating Immunostaining of Mouse Brains. J. Vis. Exp. (176), e62876, doi:10.3791/62876 (2021).
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