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08:04 min
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August 16, 2021
DOI:
1AdventHelath, 2University of Illinois Chicago School of Public Health, 3Lincoln Memorial University, 4Children’s Mercy Hospital, 5Scripps Clinic, 6Cincinnati Children’s Hospital Medical Center, 7Kansas City University, 8Roblex Rex Veterans Affairs Medical Center, 9California State University San Marcos, 10East Carolina University, 11Massachusetts Institute of Technology, 12Boehringer Ingelheim Norway KS, 13Heartland Center for Mitochondrial Medicine
Summary
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以前に凍結したアーカイブされた固体組織からのミトコンドリア濃縮サンプルを調製し、研究者は様々な実験モダリティでミトコンドリアの機能的および分析的評価を行うことを可能にした。この研究は、凍結した心臓組織からミトコンドリア濃縮製剤を調製し、ミトコンドリアの分析評価を行う方法を示す。
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Cite this Article
Barrera, D., Upton, S., Rauch, M., Notarianni, T., Eum, K. S., Liberty, M., Sah, S. V., Liu, R., Newcomer, S., May, L. E., Agbas, E., Sage, J., Kosa, E., Agbas, A. Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes. J. Vis. Exp. (174), e62809, doi:10.3791/62809 (2021).
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