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単粒子低温電子顕微鏡用生体試料の手動ブロットとプランジ凍結
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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単粒子低温電子顕微鏡用生体試料の手動ブロットとプランジ凍結

日本語

Automatically Generated

5,495 Views

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09:16 min

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February 07, 2022

DOI:

10.3791/62765-v

DOI:
10.3791/62765-v

•

09:16 min
February 07, 2022

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4 Views
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1Department of Chemistry and Biochemistry,University of California, San Diego

Summary

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この原稿は、単粒子低温電子顕微鏡用の生物学的標本を手動で凍結するブロット・アンド・プランジ法の概要を説明している。

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Cite this Article

Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, Jr., M. A. Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy. J. Vis. Exp. (180), e62765, doi:10.3791/62765 (2022).

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