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Congelamento manuale blot-and-plunge di campioni biologici per microscopia elettronica criogenica a particella singola
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Biochemistry
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JoVE Journal Biochemistry
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Congelamento manuale blot-and-plunge di campioni biologici per microscopia elettronica criogenica a particella singola

italiano

Automatically Generated

5,495 Views

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09:16 min

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February 07, 2022

DOI:

10.3791/62765-v

DOI:
10.3791/62765-v

•

09:16 min
February 07, 2022

•

4 Views
, , ,
1Department of Chemistry and Biochemistry,University of California, San Diego

Summary

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Questo manoscritto delinea il metodo blot-and-plunge per congelare manualmente i campioni biologici per la microscopia elettronica criogenica a singola particella.

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Cite this Article

Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, Jr., M. A. Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy. J. Vis. Exp. (180), e62765, doi:10.3791/62765 (2022).

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