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在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models
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在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究

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07:21 min

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June 30, 2021

DOI:

10.3791/62727-v

DOI:
10.3791/62727-v

•

07:21 min
June 30, 2021

•

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1Department of Pathobiology,University of Guelph

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Adeno-associated VirusAAVViral Vector ProductionHEK293 CellsCell StacksPreclinical StudiesLarge Animal ModelsGene TherapyHeparan SulfateCell CulturePolyethyleneimineCentrifugationClarified SupernatantCell Pellets

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Rghei, A. D., Stevens, B. A. Y., Thomas, S. P., Yates, J. G. E., McLeod, B. M., Karimi, K., Susta, L., Bridle, B. W., Wootton, S. K. Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models. J. Vis. Exp. (172), e62727, doi:10.3791/62727 (2021).

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