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低層構造状態のタンパク質検出のための高圧NMR実験
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Biochemistry
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JoVE Journal Biochemistry
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
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低層構造状態のタンパク質検出のための高圧NMR実験

日本語

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2,489 Views

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04:37 min

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June 29, 2021

DOI:

10.3791/62701-v

DOI:
10.3791/62701-v

•

04:37 min
June 29, 2021

•

5 Views
, ,
1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Summary

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高圧セルの組み立て、高圧NMR実験の設定と記録に必要なステップの詳細な説明を提供し、最後に圧力下でのピーク強度と化学シフトの両方の変化を分析します。これらの実験は、タンパク質の折りたたみ経路と構造安定性に関する貴重な洞察を提供することができます。

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Cite this Article

Nguyen, T. T., Siang, S., Roche, J. High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States. J. Vis. Exp. (172), e62701, doi:10.3791/62701 (2021).

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