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Perlas cargando proteínas y ácidos nucleicos en células humanas adherentes
JoVE Journal
Biology
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JoVE Journal Biology
Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
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Perlas cargando proteínas y ácidos nucleicos en células humanas adherentes

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4,317 Views

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07:28 min

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June 01, 2021

DOI:

10.3791/62559-v

DOI:
10.3791/62559-v

•

07:28 min
June 01, 2021

•

13 Views
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1Department of Biochemistry and Molecular Biology,Colorado State University, 2World Research Hub Initiative, Institute of Innovative Research,Tokyo Institute of Technology

Summary

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La carga de perlas introduce proteínas, plásmidos y partículas en las células de mamíferos adherentes. Esta técnica de carga celular es económica, rápida y no afecta sustancialmente la salud celular. Es más adecuado para imágenes de células vivas.

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Cite this Article

Cialek, C. A., Galindo, G., Koch, A. L., Saxton, M. N., Stasevich, T. J. Bead Loading Proteins and Nucleic Acids into Adherent Human Cells. J. Vis. Exp. (172), e62559, doi:10.3791/62559 (2021).

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