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15 di cui: la commissione per i N CPMG Relaxation Dispersion per lo studio delle dinamiche conformazionali proteiche sulla scala cronologica μs-ms
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Biochemistry
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JoVE Journal Biochemistry
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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15 di cui: la commissione per i N CPMG Relaxation Dispersion per lo studio delle dinamiche conformazionali proteiche sulla scala cronologica μs-ms

italiano

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4,688 Views

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08:09 min

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April 19, 2021

DOI:

10.3791/62395-v

DOI:
10.3791/62395-v

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08:09 min
April 19, 2021

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16 Views
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1Department of Chemistry,Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology,Iowa State University

Summary

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Qui viene fornita una descrizione dettagliata del protocollo implementato in laboratorio per l'acquisizione e l'analisi di profili di dispersione di rilassamento 15N mediante spettroscopia NMR soluzione.

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Cite this Article

Singh, A., Purslow, J. A., Venditti, V. 15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale. J. Vis. Exp. (170), e62395, doi:10.3791/62395 (2021).

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