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Immunology and Infection

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Gene Knock-in af CRISPR / Cas9 og Celle sortering i makrofag og T Cellelinjer

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Gene Knock-in af CRISPR / Cas9 og Celle sortering i makrofag og T Cellelinjer

9,722 Views

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11:32 min

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November 13, 2021

DOI:

10.3791/62328-v

DOI:

10.3791/62328-v

•

11:32 min

November 13, 2021

•

9 Views

Lichen Zhang, Rong Huang, Liaoxun Lu, Rui Fu, Guo Guo, Yanrong Gu, Zhuangzhuang Liu, Le He, Marie Malissen, Yinming Liang

¹The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, ²Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

Tags

CRISPR/Cas9 Gene Knock-in Macrophage T Cell Lines ROSA26 Locus SgRNA Design Homologous Recombination Targeting Vector Fluorescent Reporter Cell Sorting

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