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Meningitik Escherichia Coli ile Enfekte Olan Nötrofillerde Reaktif Oksijen Türlerinin Gerçek Zamanlı Nicelemesi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
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Meningitik Escherichia Coli ile Enfekte Olan Nötrofillerde Reaktif Oksijen Türlerinin Gerçek Zamanlı Nicelemesi

Türkçe

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4,187 Views

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11:00 min

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April 20, 2021

DOI:

10.3791/62314-v

DOI:
10.3791/62314-v

•

11:00 min
April 20, 2021

•

3 Views
, ,
1Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology,Ministry of Education, China Medical University

Summary

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Escherichia coli, yenidoğan Gram-negatif bakteriyel menenjitin önde gelen nedenidir. Bakteriyel enfeksiyon sırasında, nötrofiller tarafından üretilen reaktif oksijen türleri önemli bir bakterisidal rol oynar. Burada, menenjit E. coli'yeyanıt olarak nötrofillerdeki reaktif oksijen türlerini tespit etmek için bir yöntem sunuyoruz.

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Zhang, X., An, M., Zhao, W. Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli. J. Vis. Exp. (170), e62314, doi:10.3791/62314 (2021).

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