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Cuantificación en tiempo real de especies reactivas de oxígeno en neutrófilos infectados con escherichia coli meningítica
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
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Cuantificación en tiempo real de especies reactivas de oxígeno en neutrófilos infectados con escherichia coli meningítica

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4,187 Views

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11:00 min

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April 20, 2021

DOI:

10.3791/62314-v

DOI:
10.3791/62314-v

•

11:00 min
April 20, 2021

•

3 Views
, ,
1Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology,Ministry of Education, China Medical University

Summary

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La escherichia coli es la principal causa de meningitis bacteriana gram-negativa neonatal. Durante la infección bacteriana, las especies reactivas de oxígeno producidas por neutrófilos desempeñan un papel bactericida importante. Aquí introducimos un método para detectar las especies reactivas de oxígeno en neutrófilos en respuesta a la meningitis E. coli.

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Zhang, X., An, M., Zhao, W. Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli. J. Vis. Exp. (170), e62314, doi:10.3791/62314 (2021).

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