Journal
/
Immunology and Infection
/
Quantificazione in tempo reale delle specie reattive dell'ossigeno nei neutrofili infettati da Escherichia Coli meningitica
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
Please note that all translations are automatically generated. Click here for the English version.

Quantificazione in tempo reale delle specie reattive dell'ossigeno nei neutrofili infettati da Escherichia Coli meningitica

italiano

Automatically Generated

4,187 Views

•

11:00 min

•

April 20, 2021

DOI:

10.3791/62314-v

DOI:
10.3791/62314-v

•

11:00 min
April 20, 2021

•

3 Views
, ,
1Department of Developmental Cell Biology, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology,Ministry of Education, China Medical University

Summary

Automatically generated

Escherichia coli è la principale causa di meningite batterica gram-negativa neonatale. Durante l'infezione batterica, le specie reattive di ossigeno prodotte dai neutrofili svolgono un ruolo battericida importante. Qui introduciamo un metodo per rilevare le specie reattive dell'ossigeno nei neutrofili in risposta alla meningite E. coli.

Explore More Videos

Reactive Oxygen SpeciesNeutrophilsEscherichia ColiMeningitisNeonatal Bacterial MeningitisBacteremiaBlood-brain BarrierHost DefenseROS ProductionBacterial ColonyBrain-Heart Infusion MediumRifampicinPeripheral Blood SamplesRed Blood Cell LysisMagnetic Cell SortingWhite Blood CellsNeutrophils

Read Article

Cite this Article

Zhang, X., An, M., Zhao, W. Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli. J. Vis. Exp. (170), e62314, doi:10.3791/62314 (2021).

Download .ris file

Copy

Share Video

.

Copy