In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche.
Hüttner, S. S., Hayn, C., Ahrens, H. E., Schmidt, M., Henze, H., von Maltzahn, J. Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo. J. Vis. Exp. (168), e62257, doi:10.3791/62257 (2021).