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Métodos de microscopia em escala de tempo para a caracterização de microbolhas fluorescentes para liberação de medicamentos acionados por ultrassom
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Métodos de microscopia em escala de tempo para a caracterização de microbolhas fluorescentes para liberação de medicamentos acionados por ultrassom

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3,467 Views

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06:02 min

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June 12, 2021

DOI:

10.3791/62251-v

DOI:
10.3791/62251-v

•

06:02 min
June 12, 2021

•

26 Views
, , , , , , ,
1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center,University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center,University of Twente, 3Department of Biotechnology and Nanomedicine,SINTEF Industry, 4Department of Circulation and Medical Imaging,Norwegian University of Science and Technology, 5Department of Health Research,SINTEF Digital, 6Cancer Clinic,St. Olav’s Hospital, 7Department of Physics,Norwegian University of Science and Technology

Summary

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Os protocolos apresentados podem ser usados para caracterizar a resposta de microbolhas fluorescentes rotuladas projetadas para aplicações de entrega de medicamentos acionados por ultrassom, incluindo seus mecanismos de ativação, bem como seus efeitos biológicos. Este artigo abrange uma gama de técnicas de microscopia in vitro e in vivo realizadas para capturar o comprimento e escalas de tempo relevantes.

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MicrobubblesUltrasoundDrug DeliveryFluorescence MicroscopyBrightfield MicroscopyMulti-timescaleCharacterization

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Nawijn, C., Segers, T., Lajoinie, G., Mørch, Ý., Berg, S., Snipstad, S., de Lange Davies, C., Versluis, M. Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release. J. Vis. Exp. (172), e62251, doi:10.3791/62251 (2021).

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