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Multi-timescale microscopiemethoden voor de karakterisering van fluorescerend gelabelde microbubbels voor echografie-getriggerde medicijnafgifte
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
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Multi-timescale microscopiemethoden voor de karakterisering van fluorescerend gelabelde microbubbels voor echografie-getriggerde medicijnafgifte

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3,467 Views

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06:02 min

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June 12, 2021

DOI:

10.3791/62251-v

DOI:
10.3791/62251-v

•

06:02 min
June 12, 2021

•

26 Views
, , , , , , ,
1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center,University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center,University of Twente, 3Department of Biotechnology and Nanomedicine,SINTEF Industry, 4Department of Circulation and Medical Imaging,Norwegian University of Science and Technology, 5Department of Health Research,SINTEF Digital, 6Cancer Clinic,St. Olav’s Hospital, 7Department of Physics,Norwegian University of Science and Technology

Summary

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De gepresenteerde protocollen kunnen worden gebruikt om de respons van fluorescerend gelabelde microbubbels te karakteriseren die zijn ontworpen voor toepassingen met echografie-geactiveerde medicijnafgifte, inclusief hun activeringsmechanismen en hun bio-effecten. Dit artikel behandelt een reeks in vitro en in vivo microscopietechnieken die worden uitgevoerd om de relevante lengte en tijdschalen vast te leggen.

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MicrobubblesUltrasoundDrug DeliveryFluorescence MicroscopyBrightfield MicroscopyMulti-timescaleCharacterization

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Nawijn, C., Segers, T., Lajoinie, G., Mørch, Ý., Berg, S., Snipstad, S., de Lange Davies, C., Versluis, M. Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release. J. Vis. Exp. (172), e62251, doi:10.3791/62251 (2021).

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