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CRISPR/CasRx를 사용하여 드로소필라 멜라노가스터 에서 유비쿼터스 및 조직 별 RNA 표적화
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
Please note that all translations are automatically generated. Click here for the English version.

CRISPR/CasRx를 사용하여 드로소필라 멜라노가스터 에서 유비쿼터스 및 조직 별 RNA 표적화

English

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2,646 Views

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06:37 min

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February 05, 2021

DOI:

10.3791/62154-v

DOI:
10.3791/62154-v

•

06:37 min
February 05, 2021

•

13 Views
, , , ,
1Division of Biological Sciences, Section of Cell and Developmental Biology,University of California

Summary

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이 문서에서는 RNA 표적 Cas13D 효소(RfxCas13D)를 파리에서 사용하기 위한 상세한 프로토콜을 간략하게 설명합니다.

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CRISPRCasRxRNA TargetingDrosophila MelanogasterUbiquitousGene Transcript ReductionTwo-component SystemThree-component SystemGRNAUbiq CasRxLuciferasePhenotypeMendelian Genetics

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Sun, R., Brogan, D., Buchman, A., Yang, T., Akbari, O. S. Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx. J. Vis. Exp. (168), e62154, doi:10.3791/62154 (2021).

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