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Investigando a calcificação da válvula aórtica através do isolamento e cultura de linfócitos T usando células alimentadoras do casaco de buffy irradiado
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February 04, 2021
DOI:
1Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine,University of Florence, 3Berlin Institute of Health, 4Department of Cardiology,German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology,Helios Klinikum Erfurt
Summary
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Neste estudo, descrevemos o processo de isolamento de linfócitos T a partir de amostras frescas de válvulas aórticas calcificadas e as etapas analíticas da clonagem de células T para a caracterização dos subconjuntos leucócitos adaptativos usando a análise de citometria de fluxo.
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Curini, L., Christopher, M. R., Grubitzsch, H., Landmesser, U., Amedei, A., Lauten, A., Alushi, B. Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat. J. Vis. Exp. (168), e62059, doi:10.3791/62059 (2021).
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