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February 04, 2021
DOI:
1Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine,University of Florence, 3Berlin Institute of Health, 4Department of Cardiology,German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology,Helios Klinikum Erfurt
Summary
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本研究では、石灰化大動脈弁の新鮮なサンプルからのTリンパ球分離のプロセスと、フローサイトメトリー分析を用いた適応性白血球サブセットの特性評価のためのT細胞クローニングの分析ステップについて述べた。
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Cite this Article
Curini, L., Christopher, M. R., Grubitzsch, H., Landmesser, U., Amedei, A., Lauten, A., Alushi, B. Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat. J. Vis. Exp. (168), e62059, doi:10.3791/62059 (2021).
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