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Verwendung von Grafix zum Nachweis transienter Interaktoren von Saccharomyces cerevisiae Spliceosome Subcomplexes
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Biochemistry
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JoVE Journal Biochemistry
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
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Verwendung von Grafix zum Nachweis transienter Interaktoren von Saccharomyces cerevisiae Spliceosome Subcomplexes

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3,316 Views

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05:44 min

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November 09, 2020

DOI:

10.3791/61994-v

DOI:
10.3791/61994-v

•

05:44 min
November 09, 2020

•

8 Views
, , , ,
1Department of Biochemistry, Institute of Chemistry,University of Sao Paulo

Summary

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Hier beschreiben wir die Verwendung von Grafix (Gradient Fixation), einer Glyceringradientenzentrifugation in Gegenwart eines Vernetzers, um Wechselwirkungen zwischen Spleißfaktoren zu identifizieren, die vorübergehend an den Spleißosomkomplex binden.

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Cite this Article

Carvalho, F. A., Barros, M. R. A., Girotto, T. P. F., Perona, M. G., Oliveira, C. C. Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes. J. Vis. Exp. (165), e61994, doi:10.3791/61994 (2020).

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