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Complementação da Atividade de Emenda por um Complexo Galectin-3 - U1 snRNP on Beads
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Biochemistry
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JoVE Journal Biochemistry
Complementation of Splicing Activity by a Galectin-3 – U1 snRNP Complex on Beads

Complementação da Atividade de Emenda por um Complexo Galectin-3 - U1 snRNP on Beads

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08:48 min

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December 09, 2020

DOI:

10.3791/61990-v

DOI:
10.3791/61990-v

•

08:48 min
December 09, 2020

•

1 Views
, , ,
1Department of Biochemistry and Molecular Biology,Michigan State University, 2Department of Microbiology and Molecular Genetics,Michigan State University

Summary

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Este artigo descreve os procedimentos experimentais para (a) esgotamento do U1 snRNP a partir de extratos nucleares, com perda concomitante de atividade de emenda; e (b) reconstituição da atividade de emenda no extrato empobrecido U1 por partículas de galectina-3 - U1 snRNP ligadas a contas covalentemente acopladas a anticorpos anti-galectina-3.

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Cite this Article

Voss, P. G., Haudek, K. C., Patterson, R. J., Wang, J. L. Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads. J. Vis. Exp. (166), e61990, doi:10.3791/61990 (2020).

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