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酶的结晶和结构确定：多功能微流体芯片中串行晶体学的基板复合物
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
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酶的结晶和结构确定：多功能微流体芯片中串行晶体学的基板复合物

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10:45 min

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March 20, 2021

DOI:

10.3791/61972-v

DOI:
10.3791/61972-v

•

10:45 min
March 20, 2021

•

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1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS,Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry,Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

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CrystallizationStructural DeterminationEnzyme-substrate ComplexSerial CrystallographyMicrofluidic ChipCounter-diffusionIn Situ X-ray DiffractionBiomoleculeCrystal GrowthRoom Temperature Analysis

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de Wijn, R., Rollet, K., Olieric, V., Hennig, O., Thome, N., Noûs, C., Paulus, C., Lorber, B., Betat, H., Mörl, M., Sauter, C. Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip. J. Vis. Exp. (169), e61972, doi:10.3791/61972 (2021).

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