Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Poonam Sharma; Carmine Gentile

doi:10.3791/61962

Journal

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Bioengineering

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Cardiale Sferoïden als in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

JoVE Journal
Bioengineering
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JoVE Journal Bioengineering

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

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Cardiale Sferoïden als in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

7,060 Views

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10:41 min

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January 23, 2021

DOI:

10.3791/61962-v

DOI:

10.3791/61962-v

•

10:41 min

January 23, 2021

•

11 Views

Poonam Sharma^1,2,3,4, Carmine Gentile^2,3,4

¹University of Newcastle, ²University of Sydney, ³Kolling Institute of Medical Research,Royal North Shore Hospital, ⁴University of Technology, Sydney

Summary

Dit protocol is bedoeld om 3D-hartsferoïden (CS'en) te fabriceren door cellen in hangende druppels te co-culturing. Collageen-ingebedde ES's worden behandeld met doxorubicine (DOX, een cardiotoxisch middel) bij fysiologische concentraties om hartfalen te modelleren. In vitro testen met dox-behandelde ES's kunnen worden gebruikt om nieuwe therapieën voor patiënten met hartfalen te identificeren.

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Cardiale Sferoïden als in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

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Summary

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